HOW IS PREIMPLANTATION GENETIC DIAGNOSIS (PGD) PERFORMED?

PGD, or pre-implantation genetic diagnosis, is used to check an embryo prior to use within a Denver IVF cycle for any potential genetic defects present. There are three separate stages of embryo progression in which the testing is done, each with a unique purpose for testing at that stage.

A POLAR BODY BIOPSYPGD testing

The polar bodies of an embryo are the products of egg division. These cells serve no actual purpose in either the egg or the embryo, and if left alone will degrade naturally over time. They will provide the current genetic status of the egg if they are tested.

The upside of using the polar bodies is that when tested in tandem, they provide a more accurate representation of the chromosomal composition of the embryo when compared to blastomere biopic testing.  This testing occurs at either day 0 or day 1 of growth and both polar bodies are removed for testing.

One negative about performing the test this early is that at this stage in development, you will only be able to test for maternal genetic contributions. For those couples who do not have the option of discarding a fertilized embryo, it is possible to place this cell cluster into cryopreservation prior to the fusion of egg and sperm.

BLASTOMERE BIOPSY

Cells for this testing are derived from a day 3 embryo that is in the cleavage division stage. Typically at this point, there are between 6 and 8 cells available for testing. By this time, these cells have both maternal and paternal genetic material within them. This is the most common method of testing used, but is not always the most accurate representation of the current genetic content contained within the embryo. This is because the cells potentially have differing numbers of chromosomes at this point in time, as chromosomal mosaicism is at its highest in day 3.

BLASTOCYST (TROPHECTODERM) BIOPSY

Best Fertility DoctorsThis is testing performed on the fifth or sixth day of development after the embryo has reached a specific stage. There are two cell types at this age: an inner cell mass that develops in the fetal tissues, and the outer lining of cells encompassing this mass that eventually grow into the placenta. A small number of these outer lining cells are removed for testing and provide a significantly reduced chance of chromosomal mosaicism, as well as providing DNA amplification to provide for accurate testing.

You Might Also Enjoy...

Myths and Facts About IVF

Couples struggling with infertility may consider in vitro fertilization (IVF) as a possible means of achieving their dream of becoming parents. Unfortunately, many myths still surround this increasingly popular procedure used to help women conceive.

Egg Freezing: What You Need to Know

What can you do if you’re not yet ready to be a parent but want to make sure you can beat the tick-tock of that infamous biological clock that counts down your fertile years? Discover more about egg freezing and why many women are considering it.

The Effects of Holiday Stress on Your Fertility

Regardless of what else is going on in the world, the holidays bring their own brand of stress. Find out what holiday stress may mean for your fertility. More importantly, learn from an expert how to help deal with stress over the Holiday season.

What Is Preimplantation Genetic Testing?

Miracles can happen when you combine advanced medical technology with the expertise of a renowned Fertility Specialist. Learn how preimplantation genetic testing can help turn daydreams into reality for couples struggling with infertility.

8 Symptoms of Endometriosis

Endometriosis may cause excessive menstrual bleeding, nausea, or severe pelvic pain. Or it might not. You may not even know you have it until you decide to have a child. Find out more about endometriosis and how it can affect fertility.